Assembling an ion channel: ORF 3a from SARS‐CoV
Identifieur interne : 000B43 ( Main/Exploration ); précédent : 000B42; suivant : 000B44Assembling an ion channel: ORF 3a from SARS‐CoV
Auteurs : Tze-Hsiang Chien [Taïwan] ; Ya-Ling Chiang [Taïwan] ; Chin-Pei Chen [Taïwan] ; Petra Henklein [Allemagne] ; Karen H Nel [Allemagne] ; Ing-Shouh Hwang [Taïwan] ; Dieter Willbold [Allemagne] ; Wolfgang B. Fischer [Taïwan]Source :
- Biopolymers [ 0006-3525 ] ; 2013-09.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Double couche lipidique.
- Canaux ioniques, Séquence d'acides aminés, Virus du SRAS.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Lipid Bilayers.
- Teeft :
- A_id, Amino Acid Sequence, Amino acids, Assembly process, Bilayer, Bilayer recordings, Biochim biophys acta, Biol, Biol chem, Biophys, Biopolymers, Buffer conditions, Cadmus, Calcium buffer, Channel, Channel activity, Channel protein, Channel recording data, Channel recordings, Chem, Conductance, Conductance levels, Conductance patterns, Conductance states, Contract grant sponsor, Customer a_id, Experimental data, Febs lett, Fischer, Full length, Full length protein, Fusion protein, Gating kinetics, Individual tmds, Ion Channels, J_id, Linear regression analysis, Lipid, Lipid bilayer, Lipid bilayers, Lipid membrane, Lipid membranes, Manoharj, Manoharj time, Middle panel, Monomer, Negative voltages, Nicotinic acetylcholine, Peptide, Polytopic channel proteins, Pore, Positive voltage, Potassium buffer, Potassium channel, Potassium ions, Present study, Proc natl acad, Protein expression, Reconstituted, Room temperature, SARS Virus, Selectivity, Stock solution, Tetrameric channel, Tmd1, Tmd2, Tmd3, Tmds, Viral channel, Wang, Weight ratio, Wiley periodicals.
Abstract
Protein 3a is a 274 amino acid polytopic channel protein with three putative transmembrane domains (TMDs) encoded by severe acute respiratory syndrome corona virus (SARS‐CoV). Synthetic peptides corresponding to each of its three individual transmembrane domains (TMDs) are reconstituted into artificial lipid bilayers. Only TMD2 and TMD3 induce channel activity. Reconstitution of the peptides as TMD1 + TMD3 as well as TMD2 + TMD3 in a 1 : 1 mixture induces membrane activity for both mixtures. In a 1 : 1 : 1 mixture, channel like behavior is almost restored. Expression of full length 3a and reconstitution into artificial lipid bilayers reveal a weak cation selective (PK ≈ 2 PCl) rectifying channel. In the presence of nonphysiological concentration of Ca‐ions the channel develops channel activity. © 2013 Wiley Periodicals, Inc. Biopolymers 99:628–635, 2013.
Url:
DOI: 10.1002/bip.22230
Affiliations:
- Allemagne, Taïwan
- Berlin, District de Cologne, District de Düsseldorf, Rhénanie-du-Nord-Westphalie
- Berlin, Düsseldorf, Juliers
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000327
- to stream Istex, to step Curation: 000307
- to stream Istex, to step Checkpoint: 000055
- to stream PubMed, to step Corpus: 000657
- to stream PubMed, to step Curation: 000657
- to stream PubMed, to step Checkpoint: 000651
- to stream Ncbi, to step Merge: 000319
- to stream Ncbi, to step Curation: 000319
- to stream Ncbi, to step Checkpoint: 000319
- to stream Main, to step Merge: 000B45
- to stream Main, to step Curation: 000B43
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Assembling an ion channel: ORF 3a from SARS‐CoV</title><author><name sortKey="Chien, Tze Siang" sort="Chien, Tze Siang" uniqKey="Chien T" first="Tze-Hsiang" last="Chien">Tze-Hsiang Chien</name></author><author><name sortKey="Chiang, Ya Ing" sort="Chiang, Ya Ing" uniqKey="Chiang Y" first="Ya-Ling" last="Chiang">Ya-Ling Chiang</name></author><author><name sortKey="Chen, Chin Ei" sort="Chen, Chin Ei" uniqKey="Chen C" first="Chin-Pei" last="Chen">Chin-Pei Chen</name></author><author><name sortKey="Henklein, Petra" sort="Henklein, Petra" uniqKey="Henklein P" first="Petra" last="Henklein">Petra Henklein</name></author><author><name sortKey="H Nel, Karen" sort="H Nel, Karen" uniqKey="H Nel K" first="Karen" last="H Nel">Karen H Nel</name></author><author><name sortKey="Hwang, Ing Houh" sort="Hwang, Ing Houh" uniqKey="Hwang I" first="Ing-Shouh" last="Hwang">Ing-Shouh Hwang</name></author><author><name sortKey="Willbold, Dieter" sort="Willbold, Dieter" uniqKey="Willbold D" first="Dieter" last="Willbold">Dieter Willbold</name></author><author><name sortKey="Fischer, Wolfgang B" sort="Fischer, Wolfgang B" uniqKey="Fischer W" first="Wolfgang B." last="Fischer">Wolfgang B. Fischer</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:AC2636A6D505B30CB630996CFA9C594A87F7561A</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1002/bip.22230</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-12XH5FZK-K/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000327</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000327</idno><idno type="wicri:Area/Istex/Curation">000307</idno><idno type="wicri:Area/Istex/Checkpoint">000055</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000055</idno><idno type="wicri:doubleKey">0006-3525:2013:Chien T:assembling:an:ion</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:23483519</idno><idno type="wicri:Area/PubMed/Corpus">000657</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000657</idno><idno type="wicri:Area/PubMed/Curation">000657</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000657</idno><idno type="wicri:Area/PubMed/Checkpoint">000651</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000651</idno><idno type="wicri:Area/Ncbi/Merge">000319</idno><idno type="wicri:Area/Ncbi/Curation">000319</idno><idno type="wicri:Area/Ncbi/Checkpoint">000319</idno><idno type="wicri:Area/Main/Merge">000B45</idno><idno type="wicri:Area/Main/Curation">000B43</idno><idno type="wicri:Area/Main/Exploration">000B43</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Assembling an ion channel: ORF 3a from SARS‐CoV</title><author><name sortKey="Chien, Tze Siang" sort="Chien, Tze Siang" uniqKey="Chien T" first="Tze-Hsiang" last="Chien">Tze-Hsiang Chien</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Biophotonics, School of Biomedical Science and Engineering, National Yang‐Ming University, 112, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chiang, Ya Ing" sort="Chiang, Ya Ing" uniqKey="Chiang Y" first="Ya-Ling" last="Chiang">Ya-Ling Chiang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Physics, Academia Sinica, Nankang115, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Material Science and Engineering, National Tsing Hua University, 300, Hsinchu</wicri:regionArea><wicri:noRegion>Hsinchu</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Chin Ei" sort="Chen, Chin Ei" uniqKey="Chen C" first="Chin-Pei" last="Chen">Chin-Pei Chen</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Biophotonics, School of Biomedical Science and Engineering, National Yang‐Ming University, 112, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Henklein, Petra" sort="Henklein, Petra" uniqKey="Henklein P" first="Petra" last="Henklein">Petra Henklein</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Biochemistry, Charité‐Universitätsmedizin, Virchowweg 6, 10117, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="H Nel, Karen" sort="H Nel, Karen" uniqKey="H Nel K" first="Karen" last="H Nel">Karen H Nel</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Structural Biochemistry (ICS‐6), Research Center Jülich, Jülich</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Juliers</settlement></placeName></affiliation></author><author><name sortKey="Hwang, Ing Houh" sort="Hwang, Ing Houh" uniqKey="Hwang I" first="Ing-Shouh" last="Hwang">Ing-Shouh Hwang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Physics, Academia Sinica, Nankang115, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Material Science and Engineering, National Tsing Hua University, 300, Hsinchu</wicri:regionArea><wicri:noRegion>Hsinchu</wicri:noRegion></affiliation></author><author><name sortKey="Willbold, Dieter" sort="Willbold, Dieter" uniqKey="Willbold D" first="Dieter" last="Willbold">Dieter Willbold</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Structural Biochemistry (ICS‐6), Research Center Jülich, Jülich</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Juliers</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Physikalische Biologie, Heinrich‐Heine‐Universität Düsseldorf, Düsseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Fischer, Wolfgang B" sort="Fischer, Wolfgang B" uniqKey="Fischer W" first="Wolfgang B." last="Fischer">Wolfgang B. Fischer</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Biophotonics, School of Biomedical Science and Engineering, National Yang‐Ming University, 112, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Taïwan</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Biopolymers</title><title level="j" type="alt">BIOPOLYMERS: ORIGINAL RESEARCH ON BIOMOLECULES</title><idno type="ISSN">0006-3525</idno><idno type="eISSN">1097-0282</idno><imprint><biblScope unit="vol">99</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="628">628</biblScope><biblScope unit="page" to="635">635</biblScope><biblScope unit="page-count">8</biblScope><date type="published" when="2013-09">2013-09</date></imprint><idno type="ISSN">0006-3525</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-3525</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Ion Channels</term><term>Lipid Bilayers (metabolism)</term><term>SARS Virus</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Canaux ioniques</term><term>Double couche lipidique (métabolisme)</term><term>Séquence d'acides aminés</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lipid Bilayers</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Double couche lipidique</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>A_id</term><term>Amino Acid Sequence</term><term>Amino acids</term><term>Assembly process</term><term>Bilayer</term><term>Bilayer recordings</term><term>Biochim biophys acta</term><term>Biol</term><term>Biol chem</term><term>Biophys</term><term>Biopolymers</term><term>Buffer conditions</term><term>Cadmus</term><term>Calcium buffer</term><term>Channel</term><term>Channel activity</term><term>Channel protein</term><term>Channel recording data</term><term>Channel recordings</term><term>Chem</term><term>Conductance</term><term>Conductance levels</term><term>Conductance patterns</term><term>Conductance states</term><term>Contract grant sponsor</term><term>Customer a_id</term><term>Experimental data</term><term>Febs lett</term><term>Fischer</term><term>Full length</term><term>Full length protein</term><term>Fusion protein</term><term>Gating kinetics</term><term>Individual tmds</term><term>Ion Channels</term><term>J_id</term><term>Linear regression analysis</term><term>Lipid</term><term>Lipid bilayer</term><term>Lipid bilayers</term><term>Lipid membrane</term><term>Lipid membranes</term><term>Manoharj</term><term>Manoharj time</term><term>Middle panel</term><term>Monomer</term><term>Negative voltages</term><term>Nicotinic acetylcholine</term><term>Peptide</term><term>Polytopic channel proteins</term><term>Pore</term><term>Positive voltage</term><term>Potassium buffer</term><term>Potassium channel</term><term>Potassium ions</term><term>Present study</term><term>Proc natl acad</term><term>Protein expression</term><term>Reconstituted</term><term>Room temperature</term><term>SARS Virus</term><term>Selectivity</term><term>Stock solution</term><term>Tetrameric channel</term><term>Tmd1</term><term>Tmd2</term><term>Tmd3</term><term>Tmds</term><term>Viral channel</term><term>Wang</term><term>Weight ratio</term><term>Wiley periodicals</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Canaux ioniques</term><term>Séquence d'acides aminés</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">Protein 3a is a 274 amino acid polytopic channel protein with three putative transmembrane domains (TMDs) encoded by severe acute respiratory syndrome corona virus (SARS‐CoV). Synthetic peptides corresponding to each of its three individual transmembrane domains (TMDs) are reconstituted into artificial lipid bilayers. Only TMD2 and TMD3 induce channel activity. Reconstitution of the peptides as TMD1 + TMD3 as well as TMD2 + TMD3 in a 1 : 1 mixture induces membrane activity for both mixtures. In a 1 : 1 : 1 mixture, channel like behavior is almost restored. Expression of full length 3a and reconstitution into artificial lipid bilayers reveal a weak cation selective (PK ≈ 2 PCl) rectifying channel. In the presence of nonphysiological concentration of Ca‐ions the channel develops channel activity. © 2013 Wiley Periodicals, Inc. Biopolymers 99:628–635, 2013.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Taïwan</li></country><region><li>Berlin</li><li>District de Cologne</li><li>District de Düsseldorf</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Berlin</li><li>Düsseldorf</li><li>Juliers</li></settlement></list><tree><country name="Taïwan"><noRegion><name sortKey="Chien, Tze Siang" sort="Chien, Tze Siang" uniqKey="Chien T" first="Tze-Hsiang" last="Chien">Tze-Hsiang Chien</name></noRegion><name sortKey="Chen, Chin Ei" sort="Chen, Chin Ei" uniqKey="Chen C" first="Chin-Pei" last="Chen">Chin-Pei Chen</name><name sortKey="Chiang, Ya Ing" sort="Chiang, Ya Ing" uniqKey="Chiang Y" first="Ya-Ling" last="Chiang">Ya-Ling Chiang</name><name sortKey="Chiang, Ya Ing" sort="Chiang, Ya Ing" uniqKey="Chiang Y" first="Ya-Ling" last="Chiang">Ya-Ling Chiang</name><name sortKey="Fischer, Wolfgang B" sort="Fischer, Wolfgang B" uniqKey="Fischer W" first="Wolfgang B." last="Fischer">Wolfgang B. Fischer</name><name sortKey="Fischer, Wolfgang B" sort="Fischer, Wolfgang B" uniqKey="Fischer W" first="Wolfgang B." last="Fischer">Wolfgang B. Fischer</name><name sortKey="Hwang, Ing Houh" sort="Hwang, Ing Houh" uniqKey="Hwang I" first="Ing-Shouh" last="Hwang">Ing-Shouh Hwang</name><name sortKey="Hwang, Ing Houh" sort="Hwang, Ing Houh" uniqKey="Hwang I" first="Ing-Shouh" last="Hwang">Ing-Shouh Hwang</name></country><country name="Allemagne"><region name="Berlin"><name sortKey="Henklein, Petra" sort="Henklein, Petra" uniqKey="Henklein P" first="Petra" last="Henklein">Petra Henklein</name></region><name sortKey="H Nel, Karen" sort="H Nel, Karen" uniqKey="H Nel K" first="Karen" last="H Nel">Karen H Nel</name><name sortKey="Willbold, Dieter" sort="Willbold, Dieter" uniqKey="Willbold D" first="Dieter" last="Willbold">Dieter Willbold</name><name sortKey="Willbold, Dieter" sort="Willbold, Dieter" uniqKey="Willbold D" first="Dieter" last="Willbold">Dieter Willbold</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B43 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000B43 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:AC2636A6D505B30CB630996CFA9C594A87F7561A
|texte= Assembling an ion channel: ORF 3a from SARS‐CoV
}}
| This area was generated with Dilib version V0.6.33. |  |